Mechanism of acidification of the trans-Golgi network (TGN): in situ measurements of pH using retrieval of TGN38 and furin from the cell surface
N Demaurex, W Furuya, S D'Souza… - Journal of Biological …, 1998 - ASBMB
Sorting of secretory cargo and retrieval of components of the biosynthetic pathway occur at
thetrans-Golgi network (TGN). The pH within the TGN is thought to be an important
determinant of these functions. However, studies of the magnitude and regulation of the pH
of the TGN have been hampered by the lack of appropriate detection methods. This report
describes a noninvasive strategy to measure the luminal pH of the TGN in intact cells. We
took advantage of endogenous cellular mechanisms for the specific retrieval of TGN resident …
thetrans-Golgi network (TGN). The pH within the TGN is thought to be an important
determinant of these functions. However, studies of the magnitude and regulation of the pH
of the TGN have been hampered by the lack of appropriate detection methods. This report
describes a noninvasive strategy to measure the luminal pH of the TGN in intact cells. We
took advantage of endogenous cellular mechanisms for the specific retrieval of TGN resident …
