[HTML][HTML] A comprehensive method for extraction and quantitative analysis of sterols and secosteroids from human plasma

JG McDonald, DD Smith, AR Stiles, DW Russell - Journal of lipid research, 2012 - ASBMB
We describe the development of a method for the extraction and analysis of 62 sterols,
oxysterols, and secosteroids from human plasma using a combination of HPLC-MS and GC-
MS. Deuterated standards are added to 200 μl of human plasma. Bulk lipids are extracted
with methanol: dichloromethane, the sample is hydrolyzed using a novel procedure, and
sterols and secosteroids are isolated using solid-phase extraction (SPE). Compounds are
resolved on C 18 core-shell HPLC columns and by GC. Sterols and oxysterols are …