MHC interaction and T cell recognition of carbohydrates and glycopeptides.

GY Ishioka, AG Lamont, D Thomson… - … (Baltimore, Md.: 1950 …, 1992 - journals.aai.org
GY Ishioka, AG Lamont, D Thomson, N Bulbow, FC Gaeta, A Sette, HM Grey
Journal of immunology (Baltimore, Md.: 1950), 1992journals.aai.org
The T cell independence of complex polysaccharide Ag has suggested the possibility that
carbohydrates may be incapable of T cell recognition because of a failure to interact with
MHC restriction elements and/or a failure of MHC/carbohydrate complexes to interact with
and be recognized by Ag-specific TCR. We have used two approaches to obtain information
about T cell recognition of carbohydrate. First, we have determined the capacity of a series
of oligosaccharides and glycolipids to bind a murine class II MHC molecule, IAd. No …
Abstract
The T cell independence of complex polysaccharide Ag has suggested the possibility that carbohydrates may be incapable of T cell recognition because of a failure to interact with MHC restriction elements and/or a failure of MHC/carbohydrate complexes to interact with and be recognized by Ag-specific TCR. We have used two approaches to obtain information about T cell recognition of carbohydrate. First, we have determined the capacity of a series of oligosaccharides and glycolipids to bind a murine class II MHC molecule, IAd. No significant binding was observed with the 26 compounds tested, but the limitation to these studies was that there was a relatively limited collection of synthetic carbohydrate and glycolipid structures of limited complexity available for analysis. The second approach involved the study of the effect of glycosylation of a known peptide T cell epitope (OVA 323-339) on MHC binding of the peptide and on T cell recognition. Three patterns of effects were observed: 1) no effect on either binding or T cell recognition. This pattern was observed when the carbohydrate was located at residues removed from the core MHC-binding region. When the carbohydrate was located within the core MHC-binding regions, either 2) glycosylation destroyed both MHC binding and T cell recognition; or 3) glycosylation did not ablate MHC binding or T cell recognition. In this latter instance, there was evidence to indicate that the carbohydrate moiety was an important part of the antigenic determinant recognized by T cells.
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