Insulin resistance is a cellular antioxidant defense mechanism

KL Hoehn, AB Salmon… - Proceedings of the …, 2009 - National Acad Sciences
KL Hoehn, AB Salmon, C Hohnen-Behrens, N Turner, AJ Hoy, GJ Maghzal, R Stocker
Proceedings of the National Academy of Sciences, 2009National Acad Sciences
We know a great deal about the cellular response to starvation via AMPK, but less is known
about the reaction to nutrient excess. Insulin resistance may be an appropriate response to
nutrient excess, but the cellular sensors that link these parameters remain poorly defined. In
the present study we provide evidence that mitochondrial superoxide production is a
common feature of many different models of insulin resistance in adipocytes, myotubes, and
mice. In particular, insulin resistance was rapidly reversible upon exposure to agents that act …
We know a great deal about the cellular response to starvation via AMPK, but less is known about the reaction to nutrient excess. Insulin resistance may be an appropriate response to nutrient excess, but the cellular sensors that link these parameters remain poorly defined. In the present study we provide evidence that mitochondrial superoxide production is a common feature of many different models of insulin resistance in adipocytes, myotubes, and mice. In particular, insulin resistance was rapidly reversible upon exposure to agents that act as mitochondrial uncouplers, ETC inhibitors, or mitochondrial superoxide dismutase (MnSOD) mimetics. Similar effects were observed with overexpression of mitochondrial MnSOD. Furthermore, acute induction of mitochondrial superoxide production using the complex III antagonist antimycin A caused rapid attenuation of insulin action independently of changes in the canonical PI3K/Akt pathway. These results were validated in vivo in that MnSOD transgenic mice were partially protected against HFD induced insulin resistance and MnSOD+/− mice were glucose intolerant on a standard chow diet. These data place mitochondrial superoxide at the nexus between intracellular metabolism and the control of insulin action potentially defining this as a metabolic sensor of energy excess.
National Acad Sciences