Optimization and validation of a neutralizing antibody assay for HIV-1 in A3R5 cells
M Sarzotti-Kelsoe, X Daniell, CA Todd, M Bilska… - Journal of …, 2014 - Elsevier
A3R5 is a human CD4+ lymphoblastoid cell line that was engineered to express CCR5 and
is useful for the detection of weak neutralizing antibody responses against tier 2 strains of
HIV-1. Here we describe the optimization and validation of the HIV-1 neutralizing antibody
assay that utilizes A3R5 cells, performed in compliance with Good Clinical Laboratory
Practice (GCLP) guidelines. The assay utilizes Renilla luciferase-expressing replication
competent infectious molecular clones (IMC) encoding heterologous env genes from …
is useful for the detection of weak neutralizing antibody responses against tier 2 strains of
HIV-1. Here we describe the optimization and validation of the HIV-1 neutralizing antibody
assay that utilizes A3R5 cells, performed in compliance with Good Clinical Laboratory
Practice (GCLP) guidelines. The assay utilizes Renilla luciferase-expressing replication
competent infectious molecular clones (IMC) encoding heterologous env genes from …