[HTML][HTML] The platelet-derived growth factor receptor-β phosphorylates and activates G protein-coupled receptor kinase-2: a mechanism for feedback inhibition
JH Wu, R Goswami, LK Kim, WE Miller, K Peppel… - Journal of Biological …, 2005 - ASBMB
G protein-coupled receptor kinase-2 (GRK2) serine-phosphorylates the platelet-derived
growth factor receptor-β (PDGFRβ), and thereby diminishes signaling by the receptor.
Because activation of GRK2 may involve phosphorylation of its N-terminal tyrosines by c-
Src, we tested whether the PDGFRβ itself could tyrosine-phosphorylate and activate GRK2.
To do so, we used wild type (WT) and Y857F mutant PDGFRβs in HEK cells, which lack
endogenous PDGFRs. The Y857F PDGFRβ autophosphorylates normally but does not …
growth factor receptor-β (PDGFRβ), and thereby diminishes signaling by the receptor.
Because activation of GRK2 may involve phosphorylation of its N-terminal tyrosines by c-
Src, we tested whether the PDGFRβ itself could tyrosine-phosphorylate and activate GRK2.
To do so, we used wild type (WT) and Y857F mutant PDGFRβs in HEK cells, which lack
endogenous PDGFRs. The Y857F PDGFRβ autophosphorylates normally but does not …
