A subpopulation of human peripheral blood NK cells that lacks inhibitory receptors for self-MHC is developmentally immature
S Cooley, F Xiao, M Pitt, M Gleason… - Blood, The Journal …, 2007 - ashpublications.org
S Cooley, F Xiao, M Pitt, M Gleason, V McCullar, TL Bergemann, KL McQueen, LA Guethlein…
Blood, The Journal of the American Society of Hematology, 2007•ashpublications.orgHow receptor acquisition correlates with the functional maturation of natural killer (NK) cells
is poorly understood. We used quantitative real-time polymerase chain reaction (PCR)
assays to compare NKG2 and killer immunoglobulin-like receptor (KIR) gene expression in
NK cells from allogeneic transplant recipients and their donors. Marked differences were
observed in the NK subsets of recipients who had 8-fold more CD56bright cells, diminished
KIR expression (except 2DL4), and increased NKG2A. In normal blood not all CD56dim …
is poorly understood. We used quantitative real-time polymerase chain reaction (PCR)
assays to compare NKG2 and killer immunoglobulin-like receptor (KIR) gene expression in
NK cells from allogeneic transplant recipients and their donors. Marked differences were
observed in the NK subsets of recipients who had 8-fold more CD56bright cells, diminished
KIR expression (except 2DL4), and increased NKG2A. In normal blood not all CD56dim …
Abstract
How receptor acquisition correlates with the functional maturation of natural killer (NK) cells is poorly understood. We used quantitative real-time polymerase chain reaction (PCR) assays to compare NKG2 and killer immunoglobulin-like receptor (KIR) gene expression in NK cells from allogeneic transplant recipients and their donors. Marked differences were observed in the NK subsets of recipients who had 8-fold more CD56bright cells, diminished KIR expression (except 2DL4), and increased NKG2A. In normal blood not all CD56dim cells express KIR, and a novel subpopulation of cells committed to the NK-cell lineage was defined. These cells, which comprise 19.4% ± 2.8% of the CD56dim NK population in healthy donors, express the activating NKG2D and NKG2E receptors but no KIR or NKG2A. Although the CD56dim NKG2A− KIR− NK cells lack “at least one” inhibitory receptor for autologous MHC class I, they are not fully responsive, but rather functionally immature cells with poor cytotoxicity and IFN-γ production. Upon culture with IL-15 and a stromal cell line, CD56dim and CD56bright KIR− NK cells proliferate, express KIR, and develop cytotoxicity and cytokine-producing potential. These findings have implications for the function of NK cells reconstituting after transplantation and support a model for in vivo development in which CD56bright cells precede CD56dim cells.
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