Differential measurement of constitutive (COX-1) and inducible (COX-2) cyclooxygenase expression in human umbilical vein endothelial cells using specific …
C Créminon, A Habib, J Maclouf, P Pradelles… - … et Biophysica Acta (BBA …, 1995 - Elsevier
We have produced and characterized monoclonal antibodies (mAbs) directed against a
specific carboxyterminal sequence of human cyclooxygenase-2 (residues 580–598). A
rabbit polyclonal antiserum was also raised against another sequence of 10 amino acids
(residues 570–581) not present in human constitutive cyclooxygenase-1. Affinity-purified
polyclonal antibodies, coated on microtiter plates, were used as capture antibodies in a two-
site immunometric assay, with an mAb-acetylcholinesterase conjugate used as tracer. The …
specific carboxyterminal sequence of human cyclooxygenase-2 (residues 580–598). A
rabbit polyclonal antiserum was also raised against another sequence of 10 amino acids
(residues 570–581) not present in human constitutive cyclooxygenase-1. Affinity-purified
polyclonal antibodies, coated on microtiter plates, were used as capture antibodies in a two-
site immunometric assay, with an mAb-acetylcholinesterase conjugate used as tracer. The …
