Purification of natural killer cell cytotoxic granules for assaying target cell apoptosis
JE Davis, VR Sutton, KA Browne, JA Trapani - Journal of immunological …, 2003 - Elsevier
We compared two methods originally devised to purify cytoplasmic granules from
granulocytes for their capacity to produce cytotoxic granules from natural killer cell lines,
suitable for use in target cell apoptosis assays. Both methods utilised nitrogen cavitation to
efficiently lyse cells, followed by density gradient fractionation on Percoll to separate the
granules from other organelles and granule debris. The first method, originally described by
Millard and colleagues, employed DNase I to reduce the viscosity of the initial cell lysate, but …
granulocytes for their capacity to produce cytotoxic granules from natural killer cell lines,
suitable for use in target cell apoptosis assays. Both methods utilised nitrogen cavitation to
efficiently lyse cells, followed by density gradient fractionation on Percoll to separate the
granules from other organelles and granule debris. The first method, originally described by
Millard and colleagues, employed DNase I to reduce the viscosity of the initial cell lysate, but …
