[HTML][HTML] Substrate binding of gelatinase B induces its enzymatic activity in the presence of intact propeptide
GA Bannikov, TV Karelina, IE Collier, BL Marmer… - Journal of Biological …, 2002 - ASBMB
Expression of gelatinase B (matrix metalloprotease 9) in human placenta is developmentally
regulated, presumably to fulfill a proteolytic function. Here we demonstrate that gelatinolytic
activity in situ, in tissue sections of term placenta, is co-localized with gelatinase B. Judging
by molecular mass, however, all the enzyme extracted from this tissue was found in a
proform. To address this apparent incongruity, we examined the activity of gelatinase B
bound to either gelatin-or type IV collagen-coated surfaces. Surprisingly, we found that upon …
regulated, presumably to fulfill a proteolytic function. Here we demonstrate that gelatinolytic
activity in situ, in tissue sections of term placenta, is co-localized with gelatinase B. Judging
by molecular mass, however, all the enzyme extracted from this tissue was found in a
proform. To address this apparent incongruity, we examined the activity of gelatinase B
bound to either gelatin-or type IV collagen-coated surfaces. Surprisingly, we found that upon …
