Upstream and intron regulatory regions for expression of the rat neuron-specific enolase gene
K Sakimura, E Kushiya, A Ogura, Y Kudo… - Molecular brain …, 1995 - Elsevier
Neuron-specific enolase (NSE) occurs in mature neurons and paraneurons. We have
isolated the genomic clone coding for rat NSE and clarified its gene structure. In order to
analyze the regulatory sequence in the 5'-upstream region and introns, we carried out
transient expression experiments of NSE genomic DNA fragments fused to chloramphenicol
acetyltransferase (CAT) gene which were transfected into several cultured cells. The used
cells were primary cultured rat neurons, PC12, neuroblastoma 35, neuroblastoma 103, C6 …
isolated the genomic clone coding for rat NSE and clarified its gene structure. In order to
analyze the regulatory sequence in the 5'-upstream region and introns, we carried out
transient expression experiments of NSE genomic DNA fragments fused to chloramphenicol
acetyltransferase (CAT) gene which were transfected into several cultured cells. The used
cells were primary cultured rat neurons, PC12, neuroblastoma 35, neuroblastoma 103, C6 …
