[HTML][HTML] Polyunsaturated fatty acids inhibit the expression of the glucose-6-phosphate dehydrogenase gene in primary rat hepatocytes by a nuclear posttranscriptional …
LP Stabile, SA Klautky, SM Minor, LM Salati - Journal of lipid research, 1998 - ASBMB
Expression of the glucose-6-phosphate dehydrogenase (G6PD) gene is inhibited by the
addition of polyunsaturated fatty acids to the medium of primary hepatocytes in culture. To
define the regulated step, we measured the abundance of G6PD mRNA both in the nucleus
and in total RNA and measured the transcriptional activity of the G6PD gene. Insulin and
glucose stimulated a 5-to 7-fold increase in G6PD mRNA in rat hepatocytes. This increase
was attenuated by 60% due to the addition of arachidonic acid. These changes in mRNA …
addition of polyunsaturated fatty acids to the medium of primary hepatocytes in culture. To
define the regulated step, we measured the abundance of G6PD mRNA both in the nucleus
and in total RNA and measured the transcriptional activity of the G6PD gene. Insulin and
glucose stimulated a 5-to 7-fold increase in G6PD mRNA in rat hepatocytes. This increase
was attenuated by 60% due to the addition of arachidonic acid. These changes in mRNA …