[HTML][HTML] Amyloids and Are Generated Intracellularly in Cultured Human Neurons and Their Secretion Increases with Maturation (∗)
RS Turner, N Suzuki, ASC Chyung, SG Younkin… - Journal of Biological …, 1996 - ASBMB
Previous studies have demonstrated the presence of amyloid β (Aβ) in neurons (NT2N)
derived from a human embryonal carcinoma cell line (NT2) by steady state metabolic
radiolabeling and immunoprecipitation. We show here that Aβ is present intracellularly since
trypsin digestion of intact NT2N cells at 4° C did not eliminate the Aβ recovered in cell
lysates. To determine whether both Aβ and Aβ are produced intracellularly, quantitative
sandwich enzyme-linked immunosorbent assay (ELISA) was performed using COOH …
derived from a human embryonal carcinoma cell line (NT2) by steady state metabolic
radiolabeling and immunoprecipitation. We show here that Aβ is present intracellularly since
trypsin digestion of intact NT2N cells at 4° C did not eliminate the Aβ recovered in cell
lysates. To determine whether both Aβ and Aβ are produced intracellularly, quantitative
sandwich enzyme-linked immunosorbent assay (ELISA) was performed using COOH …