A rapid method to visualize von Willebrand factor multimers by using agarose gel electrophoresis, immunolocalization and luminographic detection
DR Krizek, ME Rick - Thrombosis Research, 2000 - Elsevier
A highly sensitive and rapid clinical method for the visualization of the multimeric structure of
von Willebrand Factor in plasma and platelets is described. The method utilizes submerged
horizontal agarose gel electrophoresis, followed by transfer of the von Willebrand Factor
onto a polyvinylidine fluoride membrane, and immunolocalization and luminographic
visualization of the von Willebrand Factor multimeric pattern. This method distinguishes type
1 from types 2A and 2B von Willebrand disease, allowing timely evaluation and …
von Willebrand Factor in plasma and platelets is described. The method utilizes submerged
horizontal agarose gel electrophoresis, followed by transfer of the von Willebrand Factor
onto a polyvinylidine fluoride membrane, and immunolocalization and luminographic
visualization of the von Willebrand Factor multimeric pattern. This method distinguishes type
1 from types 2A and 2B von Willebrand disease, allowing timely evaluation and …