In vivo role of caspases in excitotoxic neuronal death: generation and analysis of transgenic mice expressing baculoviral caspase inhibitor, p35, in postnatal neurons
M Tomioka, K Shirotani, N Iwata, HJ Lee, F Yang… - Molecular brain …, 2002 - Elsevier
Caspases, a family of cysteine proteases, are thought to be critical mediators of apoptosis.
To examine the role of neuronal caspases in excitotoxic neurodegeneration in vivo, we have
generated transgenic mice expressing the baculovirus protein p35, a potent viral caspase
inhibitor, using the neuron-specific calmodulin dependent kinase-II α (CaMKII-α) promoter.
The expression of p35 was confirmed by reverse transcriptase-polymerase chain reaction
(RT-PCR), Western blotting and immunohistochemistry. We analyzed caspase activation …
To examine the role of neuronal caspases in excitotoxic neurodegeneration in vivo, we have
generated transgenic mice expressing the baculovirus protein p35, a potent viral caspase
inhibitor, using the neuron-specific calmodulin dependent kinase-II α (CaMKII-α) promoter.
The expression of p35 was confirmed by reverse transcriptase-polymerase chain reaction
(RT-PCR), Western blotting and immunohistochemistry. We analyzed caspase activation …