[PDF][PDF] Colonic proteinases: increased activity in patients with ulcerative colitis

D Bustos, G Negri, JA De Paula… - MEDICINA-BUENOS …, 1998 - researchgate.net
Materials and Methods A fecal sample was obtained from each of 25 patients with ulcerative
colitis, and 10 control subjects (members of the laboratory staff) were studied. Spontaneous
feces were collected and the samples were kept at-20 C until analysed. The severity of the
disease was assessed by the Truelove index". Five hundred milligrams from each fecal
sample were homogenized in 5 ml of 0.05 mol/L tris buffer, pH 74. After