Inhibitory glutamate response on cyclic AMP formation in cultured astrocytes
A Baba, H Saga, H Hashimoto - Neuroscience letters, 1993 - Elsevier
A Baba, H Saga, H Hashimoto
Neuroscience letters, 1993•ElsevierWe examined the effects of glutamate receptor agonists on cyclic AMP (cAMP) formation in
cultured astrocytes. l-Glutamate reduced the cAMP formation induced by either isoproterenol
(IC 50 7 μM) or forskolin without affecting the basal level. Glutamate agonists reduced the
cAMP formation in astrocytes with the following rank order of potency: l-glutamate> trans-(±)-
1-aminocyclopentane-1, 3-dicarboxylic acid (t-ACPD)= quisqualate. Pretreatment of
astrocytes with pertussis toxin resulted in partial reduction of the glutamate response and a …
cultured astrocytes. l-Glutamate reduced the cAMP formation induced by either isoproterenol
(IC 50 7 μM) or forskolin without affecting the basal level. Glutamate agonists reduced the
cAMP formation in astrocytes with the following rank order of potency: l-glutamate> trans-(±)-
1-aminocyclopentane-1, 3-dicarboxylic acid (t-ACPD)= quisqualate. Pretreatment of
astrocytes with pertussis toxin resulted in partial reduction of the glutamate response and a …
We examined the effects of glutamate receptor agonists on cyclic AMP (cAMP) formation in cultured astrocytes. l-Glutamate reduced the cAMP formation induced by either isoproterenol (IC 50 7 μM) or forskolin without affecting the basal level. Glutamate agonists reduced the cAMP formation in astrocytes with the following rank order of potency: l-glutamate> trans-(±)-1-aminocyclopentane-1, 3-dicarboxylic acid (t-ACPD)= quisqualate. Pretreatment of astrocytes with pertussis toxin resulted in partial reduction of the glutamate response and a complete attenuation of the t-ACPD response. These results suggest that astrocytes have another type of metabotropic glutamate receptor which inhibits adenylate cyclase through pertussis toxin-sensitive G-proteins.
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