Transduction of dendritic cells by antigen-encoding lentiviral vectors permits antigen processing and MHC class I–dependent presentation
S Zarei, F Leuba, JF Arrighi, C Hauser… - Journal of allergy and …, 2002 - Elsevier
Background: Because antigen-presenting dendritic cells (DCs) play a major role in the
polarization of T cells, including TH2 cells involved in allergy, strategies to modify DCs
genetically are required. Objective: The purpose of this investigation was to transduce
murine bone marrow–derived DCs with lentiviral vectors encoding antigen to demonstrate
antigen processing and MHC class I–dependent presentation. Methods: Bone marrow
leukocytes were incubated with antigen-encoding lentiviral constructs and cultured with GM …
polarization of T cells, including TH2 cells involved in allergy, strategies to modify DCs
genetically are required. Objective: The purpose of this investigation was to transduce
murine bone marrow–derived DCs with lentiviral vectors encoding antigen to demonstrate
antigen processing and MHC class I–dependent presentation. Methods: Bone marrow
leukocytes were incubated with antigen-encoding lentiviral constructs and cultured with GM …
