Crawling T cell locomotion in which activated lymphocyte function–associated antigen 1 (LFA-1) integrins participate is associated with translocation of the protein kinase C-β (PKC-β) isoenzyme to the microtubule cytoskeleton. In normal T cells and T lymphoma cell lines, this type of motility is accompanied by PKC-β–sensitive cytoskeletal rearrangements and the formation of trailing cell extensions, which are supported by microtubules. Expression of PKC-β (I) and enhanced green fluorescent protein (EGFP) in nonmotile PKC-β–deficient T cells restored their locomotory behavior in response to a triggering stimulus delivered via LFA-1 and correlated directly with the degree of cell polarization. We have also shown that PKC-β (I) is a component of the tubulin-enriched LFA-1–cytoskeletal complex assembled upon LFA-1 cross-linking. These observations may have physiological equivalents at advanced (post-integrin activation) stages of lymphocyte extravasation.