A mammalian homolog of the yeast LCB1 encodes a component of serine palmitoyltransferase, the enzyme catalyzing the first step in sphingolipid synthesis
K Hanada, T Hara, M Nishijima, O Kuge… - Journal of Biological …, 1997 - ASBMB
Serine palmitoyltransferase (SPT; EC2. 3.1. 50) catalyzes the initial step dedicated to
sphingolipid biosynthesis and is thought to be a key enzyme for regulating cellular
sphingolipid content. For SPT activity, the yeast Saccharomyces cerevisiae requires two
genes, LCB1 and LCB2. We isolated mammalian LCB1 cDNA homologs from mouse and
Chinese hamster ovary (CHO) cells and an LCB2 cDNA homolog from CHO cells. The
mammalian LCB1 proteins are predicted to have about 35% amino acid identity to the yeast …
sphingolipid biosynthesis and is thought to be a key enzyme for regulating cellular
sphingolipid content. For SPT activity, the yeast Saccharomyces cerevisiae requires two
genes, LCB1 and LCB2. We isolated mammalian LCB1 cDNA homologs from mouse and
Chinese hamster ovary (CHO) cells and an LCB2 cDNA homolog from CHO cells. The
mammalian LCB1 proteins are predicted to have about 35% amino acid identity to the yeast …
