The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature
T Omura, R Sato - J biol Chem, 1964 - hero.epa.gov
CO-binding pigment, in rabbit liver microsomes is a new hemoprotein with unusual
properties. Microsomal bound P-450, when reduced, binds CO and ethyl isocyanide.
Although the CO difference spectrum of the reduced pigment is unusual for a hemoprotein,
the isocyanide difference spectrum is characteristic of a hemoprotein compound. In
microsomes P-450 is reducible by both NADH and NADPH under anaerobic conditions, but
the reduced form is very rapidly reoxidizable in the presence of molecular oxygen. When …
properties. Microsomal bound P-450, when reduced, binds CO and ethyl isocyanide.
Although the CO difference spectrum of the reduced pigment is unusual for a hemoprotein,
the isocyanide difference spectrum is characteristic of a hemoprotein compound. In
microsomes P-450 is reducible by both NADH and NADPH under anaerobic conditions, but
the reduced form is very rapidly reoxidizable in the presence of molecular oxygen. When …
