[HTML][HTML] S. typhimurium encodes an activator of Rho GTPases that induces membrane ruffling and nuclear responses in host cells
WD Hardt, LM Chen, KE Schuebel, XR Bustelo… - Cell, 1998 - cell.com
Cell, 1998•cell.com
S. typhimurium stimulates signaling pathways leading to membrane ruffling, actin
cytoskeleton rearrangements, and nuclear responses. The stimulation requires a protein
secretion system (type III) that translocates bacterial proteins into the host cell. We show that
SopE, a substrate of this secretion system, stimulates cytoskeletal reorganization and JNK
activation in a CDC42-and Rac-1-dependent manner. A λgt11 cDNA library screen for
proteins that interact with SopE identified Rac-1 and CDC42. Furthermore, purified SopE …
cytoskeleton rearrangements, and nuclear responses. The stimulation requires a protein
secretion system (type III) that translocates bacterial proteins into the host cell. We show that
SopE, a substrate of this secretion system, stimulates cytoskeletal reorganization and JNK
activation in a CDC42-and Rac-1-dependent manner. A λgt11 cDNA library screen for
proteins that interact with SopE identified Rac-1 and CDC42. Furthermore, purified SopE …
Abstract
S. typhimurium stimulates signaling pathways leading to membrane ruffling, actin cytoskeleton rearrangements, and nuclear responses. The stimulation requires a protein secretion system (type III) that translocates bacterial proteins into the host cell. We show that SopE, a substrate of this secretion system, stimulates cytoskeletal reorganization and JNK activation in a CDC42- and Rac-1-dependent manner. A λgt11 cDNA library screen for proteins that interact with SopE identified Rac-1 and CDC42. Furthermore, purified SopE was shown to stimulate GDP/GTP nucleotide exchange in several Rho GTPases in vitro, including Rac-1 and CDC42. These findings establish a paradigm for microbial stimulation of cellular responses in which the pathogen induces signaling events by directly engaging the signaling machinery within the host cell.
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