Activation of T84 cell chloride channels by carbachol involves a phosphoinositide-coupled muscarinic M3 receptor
KEJ Dickinson, RA Frizzell, MC Sekar - European Journal of Pharmacology …, 1992 - Elsevier
Muscarinic agonists stimulate Cl secretion across monolayers of the colon tumor epithelial
cell line, T84. The muscarinic receptor has been characterized in T84 cell homogenates by
radioligand binding using [3 H] N-methylscopolamine ([3 H] NMS).[3 H] NMS bound to a
single population of sites at 25° C in 100 mM NaCl, 20 mM HEPES. 10 mM MgCl 2, pH 7.4
buffer, with calculated K d= 278 (±44) pM and B max= 40 (±6) fmol/mg protein (n= 4).
Binding was reversible (diss. t 1 2= 18±3 min) and stereoselective (dexetimide K i= 0.3 nM)⪢ …
cell line, T84. The muscarinic receptor has been characterized in T84 cell homogenates by
radioligand binding using [3 H] N-methylscopolamine ([3 H] NMS).[3 H] NMS bound to a
single population of sites at 25° C in 100 mM NaCl, 20 mM HEPES. 10 mM MgCl 2, pH 7.4
buffer, with calculated K d= 278 (±44) pM and B max= 40 (±6) fmol/mg protein (n= 4).
Binding was reversible (diss. t 1 2= 18±3 min) and stereoselective (dexetimide K i= 0.3 nM)⪢ …
