[HTML][HTML] Disruption of vitamin D receptor-retinoid X receptor heterodimer formation following ras transformation of human keratinocytes
C Solomon, M Sebag, JH White, J Rhim… - Journal of Biological …, 1998 - ASBMB
A partial resistance to the growth inhibitory influence of 1, 25-dihydroxyvitamin D 3 is
apparent when immortalized keratinocytes are transformed by the rasoncogene. The vitamin
D receptor (VDR) was isolated, analyzed, and found to be identical in normal, immortalized,
andras-transformed keratinocytes. Subsequently, nuclear extracts from immortalized and ras-
transformed keratinocytes were analyzed in gel mobility shift assays utilizing labeled vitamin
D response elements or thyroid hormone response elements. A specific protein· DNA …
apparent when immortalized keratinocytes are transformed by the rasoncogene. The vitamin
D receptor (VDR) was isolated, analyzed, and found to be identical in normal, immortalized,
andras-transformed keratinocytes. Subsequently, nuclear extracts from immortalized and ras-
transformed keratinocytes were analyzed in gel mobility shift assays utilizing labeled vitamin
D response elements or thyroid hormone response elements. A specific protein· DNA …
